Original description:
- Poey y Aloy, Felipe. 1854. "Gambusia". Memorias sobre la historia natural de la Isla de Cuba, acompañadas de sumarios Latinos y extractos en Francés. La Habana (ffm04549)
Synonyms:
- Flexipenis Turner, 1963. Determiner: Rosen, Donn E, 1963
Described species included (27):
valid: 19 synonym: 8- Heterandria affinis Baird & Girard, 1853 (valid)
- Gambusia patruelis Baird & Girard, 1853 (synonym)
- Gambusia senilis Girard, 1859 (valid)
- Gambusia humilis Girard, 1859 (synonym)
- Gambusia holbrooki Girard, 1859 (valid)
- Gambusia speciosa Girard, 1859 (valid)
- Haplochilus melanops Cope, 1870 (synonym)
- Zygonectes atrilatus Jordan & Brayton, 1878 (synonym)
- Zygonectes brachypterus Cope, 1880 (synonym)
- Zygonectes inurus Jordan & Gilbert, 1882 (synonym)
- Gambusia yucatana Regan, 1914 (valid)
- Gambusia myersi Ahl, 1923 (synonym)
- Gambusia regani Hubbs, 1926 (valid)
- Gambusia panuco Hubbs, 1926 (synonym)
- Gambusia vittata Hubbs, 1926 (valid)
- Gambusia nicaraguensis sexradiatus Hubbs, 1936 (valid)
- Gambusia alvarezi Hubbs & Springer, 1957 (valid)
- Gambusia hurtadoi Hubbs & Springer, 1957 (valid)
- Gambusia longispinis Minckley, 1962 (valid)
- Gambusia marshi Minckley & Craddock, 1962 (valid)
- Gambusia atrora Rosen & Bailey, 1963 (valid)
- Gambusia krumholzi Minckley, 1963 (valid)
- Gambusia aurata Miller & Minckley, 1970 (valid)
- Gambusia amistadensis Peden, 1973 (valid)
- Gambusia eurystoma Miller, 1975 (valid)
- Gambusia zarskei Meyer, Schories & Schartl, 2010 (valid)
- Gambusia quadruncus Langerhans et al, 2012 (valid)