Original description as Gambusia regani:
- Hubbs, Carl Leavitt. 1926. "Studies of the fishes of the order Cyprinodontes. VI". Miscellaneous Publications, Museum of Zoology, University of Michigan. (16):1-86 (ffm04579)
- Gambusia panuco Hubbs, 1926, with type locality at Río Valles, Valles, San Luis Potosí, Mexico. Determiner: Miller, 2006
Conservation: Gambusia regani is evaluated by the international union for the conservation of nature in the iucn red list of threatened species as (DD) data deficient (2018). Gambusia regani is widely distributed and many parts of the distribution remain pristine, so there is little risk to the species.