Original description as Xiphophorus multilineatus:
- Rauchenberger, Mary & K.D. Kallman, and D.C. Morizot. 1990. "Monophyly and geography of the Río Pánuco Basin swordtails (Genus Xiphophorus) with descriptions of four new species". American Museum Novitates. (2975): 1-41 (ffm00052) (abstract)
Conservation: Xiphophorus multilineatus is evaluated by the international union for the conservation of nature in the iucn red list of threatened species as (DD) data deficient (2018). The habitat remains in good shape in spite of wide deforestation for cattle raising in the river area.