Considered a synonym of Gambusia affinis (Baird & Girard, 1853)
- Parenti, Lynne & J.M. Clayton, J.C. Howe. 1999. "Catalog of type specimens of Recent fishes in the National Museum of Natural History, Smithsonian Institution, 9: Family Poeciliidae (Teleostei: Cyprinodontiformes)". Smithsonian Contributions to Zoology. (604):1-22 (ffm04565)
Original description as Zygonectes atrilatus:
- Jordan, David Starr & A.W. Brayton. 1878. "Contributions to North American ichthyology. No. 3. A. On the distribution of the fishes of the Allegany region of South Carolina, Georgia, and Tennessee, with descriptions of new or little known species". Bulletin of the United States National Museum. (12):3-95 (ffm04564)