Considered a synonym of Gambusia affinis (Baird & Girard, 1853)
- Rosen, Donn E & R.M. Bailey. 1963. "The poeciliid fishes (Cyprinodontiformes), their structure, zoogeography, and systematics". Bulletin of the American Museum of Natural History. 126(1):1-176 (ffm04566)
Original description as Gambusia patruelis:
- Baird, Spencer F. & C.F. Girard. 1853. "Descriptions of new species of fishes collected by Mr. John H. Clark, on the U. S. and Mexican Boundary Survey, under Lt. Col. Jas. D. Graham". Proceedings of the Academy of Natural Sciences of Philadelphia. 6:387-390 (ffm04562)