Species described (10):
- Brachyrhaphis hartwegi Rosen & Bailey, 1963 (Brachyrhaphis), valid
- Gambusia atrora Rosen & Bailey, 1963 (Gambusia), valid
- Gambusia atzi Rosen & Gordon, 1951, synonym
- Ophisternon aenigmaticum Rosen & Greenwood, 1976 (Ophisternon), valid
- Phallichthys fairweatheri Rosen & Bailey, 1959 (Phallichthys), valid
- Xiphophorus helleri alvarezi Rosen, 1960 (Xiphophorus), valid
- Xiphophorus montezumae cortezi Rosen, 1960 (Xiphophorus), valid
- Xiphophorus variatus evelynae Rosen, 1960 (Xiphophorus), valid
- Xiphophorus milleri Rosen, 1960 (Xiphophorus), valid
- Xiphophorus pygmaeus nigrensis Rosen, 1960 (Xiphophorus), valid