Original description as Gambusia nicaraguensis sexradiatus:
- Hubbs, Carl Leavitt. 1936. "Fishes of the Yucatan Peninsula". Carnegie Institute Washington Publications. (457) pp. 157-287 (ffm00250)
Conservation: Gambusia sexradiata is evaluated by the international union for the conservation of nature in the iucn red list of threatened species as (LC) least concern (2018).